Octenidine is an antibiosis.

Octenidine浓度低于1.5 μM（0.94 μg/mL）时，15分钟内可导致测试的微生物种群减少超过99%。测试微生物中，Staphylococcus epidermidis对Octenidine最敏感，而E. coli和C. Albicans则是最不敏感的[1]。

Octenidine hydrochloride (OCT)应用于猕猴手脚皮肤时，其抗微生物活性得以保持。0.2%至1.6%的水溶奥替尼定能将常驻菌群数量降低90至99.98%[1]。每日局部使用1%Octenidine 和氯己定溶液7天后，观察到颊侧牙面上的牙菌斑评分显著降低；奥替尼定的效果优于氯己定[2]。

The bactericidal activities of OCT and CHG against Staphylococcus aureus are measured by exposing cultures to several different concentrations of each compound for various lengths of time. OCT is diluted from the 5 mM stock solution and tested in phosphate buffer at levels ranging from 0.5 to 5.0 FsM. The test is begun by adding the bacterial culture to the test solution containing OCT or CHG; samples are taken at 5, 15, 30, and 60 min afterward. The inoculum size is approximately 106 CFU/mL[1].